What is SPR analysis?
Surface plasmon resonance (SPR) binding analysis methodology is used to study molecular interactions (1, 2). SPR is an optical technique for detecting the interaction of two different molecules in which one is mobile and one is fixed on a thin gold film (1).
How does SPR assay work?
Most SPR instruments utilize a laser that shines through a prism beneath the sensor chip, generating a total-internal-reflection condition. The reflected light will have a characteristic dip in intensity at a certain angle due to the plasmonic wave, which is measured with a detector.
What is SPR sensor?
SPR technique is an optical method that uses the changes in the refractive index very close to the sensor surface caused by the binding between an analyte in solution and its ligand immobilized on the sensor surface (McDonnell, 2001). Hereafter, the basics involved in the SPR-based sensors are presented.
Why is SPR used?
Surface plasmon resonance or SPR is an optical effect that can be utilized to measure the binding of molecules in real-time without the use of labels. SPR instruments are primarily used to measure the binding kinetics and affinity of molecular interactions.
What is principal advantage of SPR?
One of the main features of the SPR is the ability to measure the interaction between your analyte and ligand without spending the time and money on expensive labelling reagents and protocols. With SPR you don’t need to wonder whether a label is affecting the native functionality or behavior of your biomolecules.
What are SPR sensors?
SPR is the basis of many standard tools for measuring adsorption of material onto planar metal (typically gold or silver) surfaces or onto the surface of metal nanoparticles. It is the fundamental principle behind many color-based biosensor applications, different lab-on-a-chip sensors and diatom photosynthesis.
What is SPR peak?
SPR is Surface Plasmon Resonance ( collective oscillation of conduction electrons of a metal) and since you are addressing nanoparticles it is generally called Localised Surface Plasmon Resonance since the oscillation is localized in nm region.
What is the role of refractive index in SPR phenomenon?
For light, this term is the refractive index. The light is seen as refracted because the photons have a different velocity in different media. In the same way, the velocity (and therefore the momentum) of the plasmons is changed when the composition of the medium changes.